antibodies against p p70s6k  (Proteintech)

Journal: Journal of Orthopaedic Surgery and Research

Article Title: Epimedium Brevicornu and Curculigo orchioides inhibit osteoclast autophagy by degrading the level of miRNA-199 to regulate the mTOR signaling pathway

doi: 10.1186/s13018-025-06043-0

Figure Lengend Snippet: EBCO upregulated mTOR/p70S6K signaling pathway proteins in osteoclasts. ( A - B ) The protein expressions of p-mTOR, mTOR, p-p70S6K and p70S6K were detected by Western blot. GAPDH and β-Actin were used as the internal controls. n = 3, * P < 0.05, ** P < 0.01. MHY1485 (2 µM): mTOR agonist. Dactolisib (10 nM): mTOR inhibitor

Article Snippet: The membranes were then blocked by 5% fat-free milk and incubated respectively with light chain 3 (LC3) A/B antibody (14,16 kDa, 1:1000; 4108, Cell Signaling Technology, USA), Beclin-1 antibody (60 kDa, 1:1000; 3495, Cell Signaling Technology, USA), phosphorylated mTOR (p-mTOR) antibody (289 kDa, 1:1000; 5536, Cell Signaling Technology, USA), mTOR antibody (289 kDa, 1:1000; 2983, Cell Signaling Technology, USA), p70S6 kinase (p70S6K) antibody (70 kDa, 1:1000; 34475, Cell Signaling Technology, USA), phosphorylated p70S6K (p-p70S6K) antibody (70 kDa, 1:1000; 9234, Cell Signaling Technology, USA), β-Actin antibody (42 kDa, 1:5000; AF7018, Affinity Biosciences, China) and anti-GAPDH (36 kDa, 1:5000; Mab5465, Multi Sciences, China) at 4°C overnight.

Techniques: Western Blot